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. 2018 Nov 2;293(52):20227–20239. doi: 10.1074/jbc.RA118.005579

Figure 2.

Figure 2.

Effect of LOC105374325 on the expression of Bax and Bak in podocytes. A, apoptosis antibody array analysis of glomerular tissues of FSGS patients (n = 3); B, IHC analysis of Bax and Bak in glomerular tissues of FSGS patients (n = 5); C, Western blot analysis of Bax and Bak in podocytes treated with ADR (n = 3); D, Western blot analysis of Bax and Bak in podocytes transfected with LOC105374325 plasmid (n = 3); E, flow cytometric analysis of apoptotic cells in podocytes transfected with Bax or Bak plasmid (n = 5); F, flow cytometric analysis of apoptotic cells in podocytes treated with ADR, Bax siRNA, and Bak siRNA (n = 5); G, flow cytometric analysis of apoptotic cells in podocytes transfected with LOC105374325 plasmid, Bax siRNA, and Bak siRNA (n = 5). For statistical analysis, one-way ANOVA with Tukey's post hoc test was used for E–G. *, p < 0.05 compared with control; #, p < 0.05 compared with podocytes treated with ADR or transfected with LOC105374325 plasmid. Bar, 20 μm.