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. 2018 Oct 24;293(52):20029–20040. doi: 10.1074/jbc.RA118.005667

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© 2018 Wang et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 7. — CprC activates the transcription of adpA by directly binding to its promoter region. A, EMSA of CprC binding to the fluorescently labeled promoter region of adpA (P_adpA). Each lane contains 50 ng of labeled probes and 1 μg of poly(dI-dC). S, unlabeled specific probe P_adpA (30-fold) was added; N, unlabeled nonspecific probe P_hrdB (30-fold) was added. B, DNase I footprinting of CprC-binding site on P_adpA (site-aA). C, the nucleotide sequence of P_adpA. Blue letters, translational start sites. Underlined red letters, binding sites of CprC. D, qRT-PCR transcriptional analysis of adpA in WT and ΔcprC. Error bars, S.D. calculated from three independent experiments. E, nikkomycin bioassays of fermentation filtrates from different strains. F, HPLC analyses of nikkomycin in different strains. WT, S. ansochromogenes 7100. ΔcprC, disruption mutant of cprC. ΔcprC/adpAoe, adpA overexpressed in ΔcprC.