Table 1.
Bacterial strains and plasmids used in this study
| Strains/plasmids | Relevant characteristics | Source/references |
|---|---|---|
| Strains | ||
| S. ansochromogenes | ||
| 7100 | WT strain | Ref. 16 |
| ΔsabA | sabA disruption mutant | This work |
| ΔsabAc | sabA complementary strain of ΔsabA | This work |
| ΔsabR1 | sabR1 disruption mutant | This work |
| ΔsabA/ΔsabR1 | sabA and sabR1 disruption mutant | This work |
| ΔsabA/ΔsabR1c | sabR1 complementary strain of ΔsabA/ΔsabR1 | This work |
| ΔsabR2 | sabR2 disruption mutant | This work |
| ΔsabA/sabR2oe | sabR2 overexpression strain of ΔsabA | This work |
| ΔcprC | cprC disruption mutant | This work |
| ΔcprCc | cprC complementary strain of ΔcprC | This work |
| ΔsabA/cprCoe | cprC overexpression strain of ΔsabA | This work |
| ΔcprC/adpAoe | adpA overexpression strain of ΔcprC | This work |
| S. coelicolor | ||
| M1146 | act−, red−, cpk−, cda−, SCP1−, SCP2− | Ref. 33 |
| MpAPD | M1146 derivative containing PhrdB-sabAPD fusion plasmid for SABs production | This work |
| PCG | M1146 derivative containing PcprC-gusA fusion plasmid | This work |
| PCMG | M1146 derivative containing PcprCM-gusA fusion plasmid | This work |
| PCG-R1 | M1146 derivative containing PcprC-gusA fusion plasmid and plasmid containing sabR1 with its own promoter | This work |
| PCG-BR1 | M1146 derivative containing PcprC-gusA fusion plasmid and PhrdB-sabR1 fusion plasmid | This work |
| PCMG-R1 | M1146 derivative containing PcprCM-gusA fusion plasmid and plasmid containing sabR1 with its own promoter | This work |
| PCMG-BR1 | M1146 derivative containing PcprCM-gusA fusion plasmid and PhrdB-sabR1 fusion plasmid | This work |
| E. coli | ||
| JM109 | F′, proA+B+, lacIq, Δ(lacZ)M15/Δ(lac-proAB), gyrA96, recA1, relA1, endA1, hsdR17 | Invitrogen |
| C41(DE3) | F−, ompT, gal dcm hsd SB (rB−, mB−) (DE3) | Lucigen |
| ET12567/pUZ8002 | dam− dcm− hsdM− pUZ8002 | Refs. 34 and 52 |
| CpAPD | C41 derivative containing pET23b::sabAPD for SABs production | This work |
| Candida albicans CGMCC2.4159 | Indicator strain for nikkomycin bioactivity | CGMCC |
| Plasmids | ||
| pSET152 | aac (3)IV, lacZ, reppMB1*attφC31, oriT | Ref. 32 |
| pKC1139 | aac (3)IV, E. coli-Streptomyces shuttle plasmid contains a Streptomyces temperature-sensitive origin of replication eptomyces temperature-sensitive origin of replication | Ref. 35 |
| pIJ10500 | HygR, a derivative of pMS82 containing φBT1 integrase gene | Ref. 32 |
| pET23b | Expression vector | Novagen |
| pGUS | Plasmid containing gusA | Ref. 36 |
| pKC1139AD | pKC1139 derivative used for disruption of sabA | This work |
| pSET152::sabA | pSET152 containing intact sabA with its putative promoter used for complement of sabA | This work |
| pET23b::sabAPD | pET23b containing intact sabAPD | This work |
| pKC1139::hsabAPD | pKC1139 containing sabAPD and the promoter PhrdB | This work |
| pKC1139R1D | pKC1139 derivative used for disruption of sabR1 | This work |
| pSET152::sabR1 | pSET152 containing intact sabR1 with its putative promoter used for complement of sabR1 | This work |
| pET23b::sabR1 | pET23b containing SabR1 coding region | This work |
| pKC1139R2D | pKC1139 derivative used for disruption of sabR2 | This work |
| pSET152::hsabR2 | pSET152 containing sabR2 and the promoter PhrdB | This work |
| pKC1139CD | pKC1139 derivative used for disruption of cprC | This work |
| pSET152::cprC | pSET152 containing intact cprC with its putative promoter used for complement of cprC | This work |
| pSET152::hcprC | pSET152 containing intact cprC and PhrdB | This work |
| pET23b::cprC | pET23b containing cprC coding region | This work |
| pIJ10500::cgusA | pIJ10500 containing gusA and the promoter PcprC | This work |
| pIJ10500::cmgusA | pIJ10500 containing gusA and the promoter PcprCM | This work |
| pSET152::hsabR1 | pSET152 containing sabR1 and PhrdB | This work |
| M13 | Cloning vector | Stratagene |
| pPsabR1 | PsabR1 was inserted into plasmid M13 | This work |
| pPsabR2 | PsabR2 was inserted into plasmid M13 | This work |
| pPsabA | PsabA was inserted into plasmid M13 | This work |
| pPadpA | PadpA was inserted into plasmid M13 | This work |
| pSET152::hadpA | pSET152 containing adpA and PhrdB | This work |