Figure 4.

CNBH domains of CNNM proteins dimerize in solution. A, gel-filtration chromatography of the CNBH domain of CNNM3. The protein elutes as dimer of 47.8 kDa relative to gel-filtration standards (γ-globulin, 158 kDa; ovalbumin, 44 kDa; myoglobin, 17 kDa). B–E, sedimentation velocity analytical ultracentrifugation experiments of CNBH domains at three protein concentrations (15, 30, and 60 μm). The CNNM3 CNBH domain sediments as a dimer at all concentrations, whereas the domains from CNNM1, CNNM2, and CNNM4 sediment as mixtures of monomer and dimer forms (see Table S2).