Figure 5.

The miR-27a-3p mimic inhibits neuronal apoptosis and microglia activation in the perihematomal area. A, TUNEL staining was performed to detect apoptotic cells in the perihematomal zone (scale bars = 33.3 μm). B, dying neurons in the perihematomal area were labeled by Fluoro-Jade B stain (scale bars = 33.3 μm). C, immunohistochemical staining of OX-42, a marker of activated microglia, in the perihematomal zone (scale bars = 33.3 μm). D–F, statistical analyses of the TUNEL apoptotic ratio (D), Fluoro-Jade B–positive cells (E), and OX-42–positive cells (F) in the perihematomal area were performed based on the above staining images (n = 5/group). *p < 0.05.