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. 2018 Oct 18;293(52):20041–20050. doi: 10.1074/jbc.RA118.001858

Figure 6.

Figure 6.

miR-27a-3p directly targets the 3′ UTR of the AQP11 transcript. A and B, 24 h after ICH, the levels of AQP11 mRNA and AQP11 protein in the perihematomal tissues were determined by real-time PCR and Western blotting, respectively (n = 6/group). β-Actin was used as an internal control. C and D, rat BMECs were isolated and transfected with miR-NC, the miR-27a-3p mimic, or the miR-27a-3p inhibitor, and the levels of AQP11 mRNA and AQP11 protein were measured 24 h after transfection. β-Actin was used as an internal control. E, sequence of AQP11_3′ UTR. F and G, alignment of miR-27a-3p with the 3′ UTR of AQP11. The miR-27a-3p binding site in the 3′ UTR of AQP11 was mutated (ACUGUGA to CUGUCCA). H, a Dual-Luciferase reporter assay was performed in BMECs to verify direct targeting of miR-27a-3p on the 3′ UTR of AQP11. The in vitro experiments were repeated three times. *, p < 0.05; **, p < 0.01.