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. 2018 Nov 2;293(52):20099–20111. doi: 10.1074/jbc.RA118.005802

Figure 1.

Figure 1.

RNF31 is indispensable for human Treg cell function. A, MFI of RNF31 expression was measured in CD4+FOXP3+ Treg cells and CD4+FOXP3− Tconv cells from PBMC of healthy donors (n = 3, *, p < 0.05). B, CD4+CD25hiCD127low (Treg cells) T cells isolated from healthy donors were stimulated with anti-CD3/CD28 beads, IL-2 (500 units) for 7 days, followed by detection of Treg purity through FOXP3 staining. C, in vitro expanded Treg cells for a knockdown assay using shRNA-bearing lentivirus, followed by detection of Treg cell function in terms of IFN-γ secretion. D, CD4+CD25lowCD127high (Tconv cells) for a knockdown assay using shRNA-bearing lentivirus, followed by detection of IFN-γ secretion. E, CTLA4 or CD25 expression were detected in in vitro expanded Treg cells transfected with shRNA-bearing lentivirus. F, in vitro suppression assay was performed in Treg cells transfected with lentivirus carrying shCK or shRNF31 constructs. G, percentage of proliferated responder T cells was assessed as shown in F (n = 3). All data represent mean ± S.D. *, p < 0.05; **, p < 0.01; ***, p < 0.01 as determined by Student's t test. NS, not significant.