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Expression and purification of the recombinant C-terminal region of the Cry1Ab protoxin in E. coli cells. The C-terminal (C-term) fragment was purified by affinity chromatography through a nickel affinity column by using the His tag provided in the pET22b cloning vector and eluted with 250 mm imidazole. The samples were analyzed by SDS-PAGE (10% acrylamide) stained with Coomassie Blue. M, molecular size marker; molecular masses are shown in kDa.
