Figure 2.

ELISA binding analysis of Cry1Ab protoxin, Cry1Ab activated toxin, and the C-terminal fragment from Cry1Ab to BBMVs purified from M. sexta midgut tissue. A, the binding of these proteins revealed with anti-Cry1Ab antibody that was raised against the activated Cry1Ab toxin. This antibody does not cross-react with the C-terminal fragment (Fig. S1). B, the binding of these proteins revealed with anti-C-terminal antibody that was raised against the purified C-terminal fragment. This antibody does not cross-react with Cry1Ab activated toxin (Fig. S1). A total of 1 μg of protein of BBMVs was bound to each of the wells of the ELISA plate. Each experiment was performed in duplicate with a total of three repetitions. Standard deviations are shown.