Figure 3.

Binding analysis of the C-terminal fragment to CAD, APN, and ALP. A, ELISA binding assays of the C-terminal fragment of Cry1Ab to the purified recombinant CR7–12 fragment, ALP, or APN expressed in E. coli cells. Each experiment was performed in duplicate with a total of three repetitions. Standard deviations are shown. B, SPR binding analyses of the C-terminal fragment to Cry1Ab receptors, performed by immobilizing the C-terminal fragment by conventional amine coupling. Sensorgrams of serial doubling dilutions of each purified receptor (CAD (CR7–12), APN, and ALP) are shown. RU, response units.