Figure 4.

Ligand blot assays of Cry1Ab protoxin, Cry1Ab activated toxin, and the C-terminal fragment from Cry1Ab to the purified recombinant CAD (CR7–12) fragment, ALP, or APN proteins expressed in E. coli cells. Shown are serial dilutions of the recombinant receptor proteins that were loaded in SDS-PAGE and transferred to PVDF membranes. These blots were used for ligand blot binding assays. The numbers under the bands represent the percentage of each band on the blot, calculated after densitometry analysis of the bands using ImageJ software and selecting one band of the protoxin bound to the lower concentration of receptor as 100% reference. Molecular masses are indicated. A, ligand blot assays of 5 nm Cry1Ab protoxin, Cry1Ab activated toxin, and the C-terminal fragment from Cry1Ab to CAD (CR7–12). B, ligand blot assays of 200 nm Cry1Ab protoxin, Cry1Ab activated toxin, and the C-terminal fragment from Cry1Ab to ALP. C, ligand blot assays of 200 nm Cry1Ab protoxin, Cry1Ab activated toxin, and the C-terminal fragment from Cry1Ab to APN. M, molecular size marker; molecular masses are shown in kDa.