Figure 6.

ELISA binding assays of Cry1Ab and Cry1Ab-mutant proteins to the purified recombinant CAD (CR12), ALP, or APN expressed in E. coli cells. The three Cry1Ab mutants (Cry1Ab-RR368–369AA, Cry1Ab-F371A, and Cry1Ab-G439D) were affected in toxicity against M. sexta larvae. A, binding of protoxin or activated toxin molecules to the purified CAD (CR12) receptor fragment. B, binding of protoxin or activated toxin molecules to purified ALP. C, binding of protoxin or activated toxin molecules to purified APN. Each experiment was performed in duplicate with a total of three repetitions. Standard deviations are shown.