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. 2018 Nov 1;293(52):20263–20272. doi: 10.1074/jbc.RA118.005101

Table 1.

Equilibrium dissociation constants (nanomolar) for interactions between wildtype and mutant Cry1Ab protoxins and activated toxins with recombinant CR12, ALP, and APN

NB, no binding.

Type of Cry1Ab CR12
ALP
APN
Protoxin Toxin Protoxin Toxin Protoxin Toxin
WT 85 ± 14 35 ± 4 28 ± 2 296 ± 3 79 ± 8 296 ± 5
RR368–9AA NB NB 22 ± 3 381 ± 36 55 ± 6 572 ± 53
F371A NB NB 16 ± 2 242 ± 48 40 ± 5 214 ± 8
G439D NB NB 14 ± 2 806 ± 88 30 ± 4 870 ± 32