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Fluorescence intensity decay parameters for N terminus (W178L) and C terminus (W7L) in native state and in presence of denaturant (D) (8 m urea)
| Mutant | τ1 (ns) (α1) | τ2 (ns) (α2) | τ3 (ns) (α3) | τm (ns) |
|---|---|---|---|---|
| N terminus | ||||
| W178L | 0.5 (0.37) | 1.7 (0.48) | 4.8 (0.15) | 1.7 ± 0.1 |
| W178L + D | 0.56 (0.26) | 1.58 (0.52) | 4.4 (0.22) | 1.9 ± 0.08 |
| C terminus | ||||
| W7L | 0.5 (0.37) | 1.7 (0.5) | 4.8 (0.13) | 1.7 ± 0.02 |
| W7L + D | 0.67 (0.31) | 1.8 (0.51) | 4.9 (0.18) | 2.0 ± 0.28 |
