Table 1.

Cells deficient in MPV17 have 43% less mitochondrial folate

Total intracellular folate in whole-cell lysates and isolated mitochondria were quantified in a L. casei microbiological assay. Whole-cell lysates: shRNA control, n = 3; MPV17 KD, n = 6; NS (p > 0.05). Mitochondria: n = 4 each group; ‡, p ≤ 0.0001. Data are shown as mean ± S.E. Statistical significance was determined by two-tailed Student's t test.

	Folates in whole cellNS	Folates in mitochondrial fraction‡
	fmol/μg protein	fmol/μg protein
MPV17 KD	107.64 ± 17.98	74.08 ± 6.11
shRNA control	100.95 ± 14.18	129.63 ± 5.29