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. 2018 Oct 25;293(52):20062–20072. doi: 10.1074/jbc.RA118.005449

Figure 2.

Figure 2.

cFLIP degrades in RNF31-deficient cells through proteasome-dependent process. A, Western blot analysis of lysates of CHX-treated (20 μg/ml) control and RNF31 silenced HeLa cells. B, Western blot analysis of HeLa cells pretreated with different dose of Z-VAD-FMK or MG132 and then stimulated with TNFα and CHX (10 ng/ml and 10 μg/ml, respectively) for the indicated periods. C, Western blot analysis of control and RNF31-silenced HeLa cells pretreated with MG132 (10 μm) for 1.5 h and then stimulated with TNFα and CHX (10 ng/ml and 10 μg/ml, respectively) for the indicated periods. D, Western blot analysis of control and RNF31-deleted Jurkat cells pretreated with Z-VAD-FMK (10 μm) or MG132 (10 μm) and then stimulated with TNFα and CHX (10 ng/ml and 10 μg/ml, respectively) for the indicated periods.