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. 2018 Oct 25;293(52):20062–20072. doi: 10.1074/jbc.RA118.005449

Figure 3.

Figure 3.

RNF31 interacts with cFLIP. A, Western blot analysis of immunoprecipitates from 293T cells transiently transfected with the indicated plasmids using anti-FLAG beads. B, Western blot analysis of immunoprecipitates from HeLa cells stably expressing FLAG-cFLIP after stimulation with TNFα (20 ng/ml) for the indicated times using anti-FLAG beads. C, Western blot analysis of immunoprecipitates from Jurkat cells after stimulation with TNFα (20 ng/ml) for the indicated times using cFLIP antibody-conjugated beads, and IgG as control. D, schematic of domains of RNF31 and cFLIP and their truncated mutants. E and F, Western blot analysis of immunoprecipitates using anti-FLAG beads and total lysates of 293T cells transfected with the plasmids encoding the indicated domains. FL, full-length; NT, N-terminal fragment; CT, C-terminal fragment; ΔRL, RBR-LDL domain-deleted fragment; RL, RBR-LDL domain; RBR, RBR domain; DED1, DED1 domain; DED1,2, DED1 and DED2 domain; ΔDED1,2, DED1 and DED2 domain-deleted fragment.