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Tracings of diaphragm muscle force during fatiguing contractions in vitro. Muscles were electrically stimulated after incubating in buffer (control) for 60 min. A, Representative recording of an entire fatigue protocol. B, Tracing from one tetanic contraction illustrating variables used to analyze muscle fatigue. dF/dt refers to the first derivative of force as a function of time. Data shown were collected at 1000 Hz.
