Figure 4.

Src/Syk orchestrates inflammatory and anti-inflammatory cytokines in microglia cells. (a) ELISA of IL-6, TNF-α, and IL-10 production in supernatant from microglia cells pretreated with Src inhibitor PP1 for 30 min and then primed with LPS for 12 h. (b) ELISA of IL-6, TNF-α, and IL-10 production in supernatant from microglia cells pretreated with Syk inhibitor R406 for 30 min and then primed with LPS for 12 h. (c) Q-PCR assay of mRNA expression of IL-6, TNF-α, and IL-10 in microglia cells pretreated with Src inhibitor PP1. Data are representative of three different experiments. Data are presented as mean ± SEM. *P < 0.01. IL: interleukin; TNF-α: tumor necrosis factor alpha; DMSO: dimethyl sulfoxide.