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. 2017 Nov 14;39(1):58–73. doi: 10.1177/0271678X17742792

Figure 4.

Figure 4.

Inhibition of Rho signaling prevents GLT1 loss in in vitro. (a–e) Hippocampal astrocytes were treated with vehicle, or thrombin (1 U/mL) in the presence or absence of increasing doses of ROCK inhibitors Y-27632 and Fasudil, for 24 h. (a) Thrombin treatment significantly increased phosphorylation of MYPT1 (pMYPT1). (b–d) Exposure to thrombin resulted in a significant decrease in GLT1 (b, c) with no significant change in GLAST (b, d). Inhibition of ROCK with Y-27632 or Fasudil prevented the decrease in GLT1 (c), but not GLAST (d) caused by thrombin. (e) Thrombin treatment significantly decreased glutamate uptake. Inhibition of ROCK with Y-27632 or Fasudil, in the presence of thrombin significantly increased glutamate uptake compared to thrombin treatment alone. *p < 0.05, **p < 0.01 compared to control; #p < 0.05 compared to thrombin alone (n = 7–12 per experiment, each performed in duplicate or triplicate).