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. 2018 Dec 24;9:3183. doi: 10.3389/fmicb.2018.03183

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Copyright © 2018 Berges, Michel, Lassek, Nuss, Beckstette, Dersch, Riedel, Sievers, Becher, Otto, Maaß, Rohde, Eckweiler, Borrero-de Acuña, Jahn, Neumann-Schaal and Jahn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Scanning/transmission electron microscopic images and motility assays of C. difficile wild type and the corresponding fur mutant. (A) CDMM agar filled glass tubes containing 0.2 μM (-Fe) and 15 μM (+Fe) iron sulfate were inoculated with wild type (wt) and the corresponding fur mutant strain and incubated anaerobically for 24 h. Scanning electron microscopy picture of C. difficile (B, right panel) and the fur (mutant B, left panel) grown in CDMM containing 0.2 μM (-Fe) and 15 μM (+Fe) iron sulfate are shown. Negative staining (C) also depicts less flagellation of the fur mutant and no detectable other appendage-like structures on the surface of C. difficile like pili or fimbriae. Bars represent 2 μm in (B), top 2 images and 1 μm in all other images.