FIGURE 5.

Growth of wild type and fur mutant growth at high iron concentration in the presence of vancomycin and polymyxin B. Growth curves of C. difficile wild type and the corresponding fur mutant in CDM medium with 15 μM iron sulfate, without (A) and with the addition of 0.3 mg vancomycin/l (B) and 150 mg polymyxin B/l (C) are shown. Black symbols are used for the wild type and green symbols for the fur mutant. Growth was monitored every 2 h in at least five independent cultivations. Standard deviations are indicate.