Figure 2.

Early passage ASCs overexpress the neurotropic protein HGF but not VEGF nor the proinflammatory cytokine IL-1β. ASCs at passage 3 that were cultured in serum-free conditions for 48 hours were compared to the control group of ASCs at passage 5. Both cells and medium were collected and analyzed at mRNA level and at protein level by qRT-PCR and by ELISA, respectively. (a) qRT-PCR analysis of HGF, VEGF, and IL-1β. (b) ELISA for HGF protein levels. Each experiment was performed a minimum of 3 samples from 3 different patients. Each experiment was performed a minimum of 3 times.