Figure 4.

ASCs rescue RPE cell death under oxidative stress. RPE cells were incubated with P3-CM or with controls comprising P5-CM or non-CM for 48 hours, followed by exposure to H₂O₂ (1 mM, 7 h). Cells were harvested and cell death was analyzed. (a, b) Necrotic cell death was determined using PI staining followed by flow cytometry analysis. (c) Cell death visualized by acridine orange and ethidium bromide staining. Live cells appear green stained by acridine orange only (×20 magnification). CM: conditioned medium. Each experiment was performed a minimum of 3 samples from 3 different patients. Each experiment was performed a minimum of 3 times.