Figure 1.

(a) Histological analysis. H&E staining and sirius red dye to highlight the collagen fibers of liver section: (A, B) healthy hepatic tissue; (C, D) fibrotic liver tissue induced by CCl₄; (E, F) liver tissue with hepatic fibrosis treated with ovothiol A. (b) Evaluation of serum levels of liver enzymes. The levels of AST, ALT, and ALP were determined in the serum from mice affected by liver fibrosis and treated with ovothiol A or control solution. Data are expressed as mean ± SD, n = 7. The significance was determined by the ANOVA and post hoc analysis: (^∗p < 0.05) and (^∗∗p < 0.01) represent significance compared to vehicle + CCl₄; (^#p < 0.05) and (^##p < 0.01) compared to nontreated (NT) healthy mice.