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. 2018 Dec 17;2018:5045734. doi: 10.1155/2018/5045734

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Copyright © 2018 Mariarita Brancaccio et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Protein expression of liver fibrosis markers. (a) A representative experiment of Western blot analysis of cytosolic extracts obtained from hepatic tissues of mice treated with ovothiol A or vehicle, after induction of liver fibrosis, compared to samples of healthy mice (NT), using antibodies specific for TGF-β, α-SMA, and TIMP1. Histograms of the densitometry analysis of protein bands obtained by Western blot for liver markers: (b) TGF-β; (c) α-SMA; and (d) TIMP1. Data were normalized for GAPDH. Data are expressed as mean ± SD, n = 7. The significance was determined by ANOVA test. (#p < 0.05) and (##p < 0.01) represent significance compared to NT; (^∗∗p < 0.01) represents significance compared to the treated with vehicle + CCl₄.