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. 2018 Dec 17;2018:9396089. doi: 10.1155/2018/9396089

Figure 9.

Figure 9

Effect of NaHS on OPA1 and DRP1 in WT mice and SIRT3 KO mice after ISO administration. Male 129S1/SvImJ (WT) and SIRT3 knockout (KO) mice at 10 weeks of age were administrated with NaHS (50 μmol/kg/d) or normal saline (NS) once daily. After 2 weeks, the mice were given isoproterenol (ISO, 60 mg/kg) by intraperitoneal injection followed by NaHS or NS administration once daily for another 2 weeks. (a–b) The myocardium of mice was stained with OPA1 and DRP1 followed by Alexa Fluor 488- or Cy3-conjugated IgG, respectively. Tissue sections were photographed with a fluorescence microscope. The nuclei were counterstained with DAPI (blue). Bar = 100 μm. (c–d) The expression of OPA1 and DRP1 in mitochondrial proteins from the myocardium of mice was measured by western blot. VDAC1 was serviced as a loading control. Plots represent the mean ± SEM; n = 6. Statistical significance: ∗∗P < 0.01 compared with WT + NS; ##P < 0.01 compared with WT + ISO; &&P < 0.01 compared with SIRT3 KO + NS.