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. 2018 Dec 17;2018:5057372. doi: 10.1155/2018/5057372

Figure 3.

Figure 3

Effects of SSM on LPS-induced protein expression of TNF-α, IL-6, iNOS, and COX-2. RAW 264.7 cells were induced with 1 μg/mL LPS and various concentrations of SSM for 24 h. (a) Relative protein expression levels of TNF-α and IL-6. (b) Relative protein expression levels of iNOS and COX-2. Bands were detected using an enhanced chemiluminescence (ECL) detection kit. Actin was used as an internal control (46 kDa). (A) Control group, (B) 1 μg/mL LPS-administered group, (C) 1 μg/mL LPS-administered and 0.1 μg/mL SSM-treated group, (D) 1 μg/mL LPS-administered and 1 μg/mL SSM-treated group, and (E) 1 μg/mL LPS-administered and 10 μg/mL SSM-treated group. The results are presented as the means ± standard errors of the mean (SEMs). ∗P<0.05 compared to the control group. #P<0.05 compared to the LPS-induced group.