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. 2018 Dec 17;2018:5057372. doi: 10.1155/2018/5057372

Figure 4.

Figure 4

Effects of SSM on LPS-induced NF-κB translocation and degradation of IκBα protein expression. The cells were induced with 1 μg/mL LPS and various concentrations of SSM for 24 h. (a) Relative protein expression of NF-κB in the nucleus. (b) Relative protein expression of IκBα in the cytosol. Bands were detected using an enhanced chemiluminescence (ECL) detection kit. Actin was used as an internal control (46 kDa). (A) Control group, (B) 1 μg/mL LPS-administered group, (C) 1 μg/mL LPS-administered and 0.1 μg/mL SSM-treated group, (D) 1 μg/mL LPS-administered and 1 μg/mL SSM-treated group, and (E) 1 μg/mL LPS-administered and 10 μg/mL SSM-treated group. The results are presented as the means ± standard errors of the mean (SEMs). ∗P<0.05 compared to the control group. #P<0.05 compared to the LPS-induced group.