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. 2016 Dec 26;26(3):599–610. doi: 10.1093/hmg/ddw419

Figure 3.

Figure 3.

Effects on mitosis of Borealin mutants. (A). G2/M phases in Nthy transfected with Borealin-wt, Borealin-114, Borealin-148 and Borealin-177. Borealin transfection in cells lead to increase the mitosis. The percentage of cells was determined by flow cytometry. Values are represented as mean ± SEM from three independent measurements. NT, non-transfected; Mock, cells transfected with empty vector; Borealin-wt, Borealin-114, Borealin-148 and Borealin-177 were cells transfected with sh-vector and with vector containing Borealin-wt or mutated. (B) Localization of Borealin mutants during metaphase. HeLa M cells were transiently transfected with GFP-tagged wild-type and mutant Borealin. Single z-planes of representative transfected cells are shown. BOR, Borealin, AURB, Aurora B kinase, ACA, anti-centromere autoantibodies. (C) Time lapse. Examples of cells progressing through mitosis after transfection with wild-type and mutant Borealin-GFP along with Borealin shRNA. (D) Length of mitosis. GFP-positive cells as described in C were tracked from prophase to telophase. Average + SEM are shown.