Figure 4: Id3^- eT_R are enriched and resident in non-lymphoid tissues.

A) Representative flow cytometry histograms and graphical analysis of expression of the CD69 and CD103 by gated splenic T_R populations. B) Enrichment of CD8⁺ T_RM (red) or circulating T cell (blue) gene sets along ranked lists of the indicated pairwise comparisons. C) Heatmap and hierarchical clustering of splenic RNA-seq samples based on ST2⁺ tissue T_R associated genes. D) Representative flow cytometry plots of CD103 and CD69 expression by gated TCRβ⁺ CD4⁺ Foxp3⁺ T_R in the indicated tissues. E) Graphical analysis of Id3^- eT_R frequency among total T_R in various tissues. F) Representative histograms of GFP expression from various tissues of Id3-GFP x Foxp3-mRFP mice, gated on TCRβ⁺ CD4⁺ Foxp3⁺ T_R.