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. 2018 Sep 20;104(1):197–206. doi: 10.3324/haematol.2018.193680

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Figure 2. — Frequencies of HLA-class II-restricted CD4 T-cell clones. (A) Frequencies (%) of T-cell clones showing reactivity against allogeneic CD14-derived dendritic cells (alloDC) and third-party Epstein-Barr virus-transformed lymphoblastoid cell lines (EBV-LCL) expressing the targeted mismatched HLA-DP allele (black bars) or against alloDC but not against third-party EBV-LCL expressing the targeted mismatched HLA-DP allele (gray bars) in responses 1-5 (n=22, 46, 429, 549, 157, respectively). Pair 5 was a 9/10 HLA match containing an additional HLA-DQ mismatch; the HLA-DP-restricted T-cell clones are shown in bar 5a and the HLA-DQ-restricted T-cell clones in bar 5b. (B) Interferon-gamma (IFNγ) production by a representative HLA-DPB1*03:01-restricted T-cell clone (from response 4) after overnight incubation with hematopoietic stimulator cells (DC, CD14⁺ cells, PHA-blasts and EBV-LCL). The clone only shows reactivity against alloDC and alloEBV-LCL. (C) Reactivity of a representative HLA-DPB1*03:01-restricted T-cell clone (from response 4) against only alloDC. (D) The representative HLA-DPB1*03:01-restricted T-cell clone from Figure 2B tested against a panel of third-party DC. (E) The representative HLA-DPB1*03:01-restricted T-cell clone from Figure 2C was also tested against third-party DC. (F) The representative clone of Figure 2B shows reactivity against a panel of third-party EBV-LCL, but (G) the clone of Figure 2C does not show reactivity against third-party EBV-LCL. The number between brackets indicates the individual from whom the stimulator cells were derived.