Extended Data Figure 6 |. vtc4⁺ and rpl3402⁺ are critical for inheritance of silencing at the endogenous ade6⁺ locus.

a, Schematic of the siRNA driver cen::ade6⁺ locus on chromosome 1 (upper) and the endogenous ade6⁺ locus (lower) in which the vt4⁺ and rpl3402⁺ genes were replaced with the ura4⁺ gene ( vtc4-rpl3402∆::ura4⁺). b, Frequency of silencing establishment at endogenous ade6⁺ in cen::ade6⁺ leo1∆ vtc4-rpl3402∆ cells. Repeated twice. c, cen::ade6⁺ leo1∆ vtc4-rpl3402∆ ade6⁺-OFF cells were crossed to cen⁺ leo1⁺ ade6^BC+-ON cells followed by random spore analysis (RSA) to test the epigenetic maintenance of the OFF state in the absence of the cen::ade6⁺ siRNA driver and the leo1∆ enabling mutation. The number of progeny matching each genotype and phenotype are shown. 80 white and 80 red progeny were genotyped. Results reflect two independent crosses.