Extended Data Figure 2 |. The acquired ade6⁺ silent allele is stable in the absence of the cen::ade6⁺ siRNA trigger and the mlo3∆ enabling mutation.

a, ade6⁺-OFF ^progeny of the cross in Fig. 2b with the indicated genotypes were plated on Low Ade medium. See Fig 2c for related results.

b-e, Biological replicates of the crosses shown in Fig. 2d-g. cen::ade6⁺ mlo3∆ ade6⁺-OFF cells were crossed to cen⁺ mlo3⁺ ade6^BC+-ON cells with deletions of key RNAi components (b-d) or H3K9 methyltransferase Clr4 (e) followed by RSA. All ade6⁺-OFF progeny were RNAi⁺ and clr4⁺. Bars indicate number of ade6⁺-OFF meiotic progeny for each genotype.