Extended Data Figure 4 |. cis inheritance of the acquired ade6⁺ silencing and its stable propagation over multiple meiotic generations.

a, ade6^BC-OFF cells crossed to ade6⁺-ON cells, followed by tetrad dissection on Low Ade medium (top) and genotyping using allele-specific PCRs (bottom), showed the 2:2 segregation of the OFF and ON states and cis transmission of each state. Performed once, but see Fig. 3a for reciprocal cross. b, ade6⁺-OFF progeny of repeated ade6⁺-OFF x ade6^BC+-ON crosses were selected and crossed again, showing stability of the ade6⁺-OFF allele over five meiotic generations. n, number of meiotic progeny analyzed. Independent replicate of cross in Fig. 3c.