Virus-specific antibodies were detected by means of ELISA and HI assays with purified Ca04 (A) or VN1203 (B) virus as a viral antigen. IgG antibody titers (upper, left panels) and HI titers (upper, right panels) in serum and IgA antibody titers in the BALF (lower, left panels), and nasal washes (lower, right panels) from mice intranasally mock-immunized with PBS or hemozoin or immunized with non-adjuvanted or hemozoin-adjuvanted inactivated virus were measured. Values represent antibody titers in individual mice (A: n=3, B: n=5). Statistically significant differences (*: P<0.05, **: P<0.01) are indicated.