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. Author manuscript; available in PMC: 2018 Dec 31.
Published in final edited form as: Curr Opin Genet Dev. 2018 Mar 26;49:85–94. doi: 10.1016/j.gde.2018.03.006

Figure 2. Important considerations for performing transposon screens.

Figure 2

Critical considerations for performing transposon screens include: 1) the selection of the transposon gene trap vector (SB lines = T2/Onc, T2/Onc2, T2/Onc3; PB lines = ATP1, ATP2, ATP3 that differ based on the transposon copy number within the concatemer and site of integration on different chromosomes), 2) the transposase line, 3) the selection of the Cre driver line used to induce expression of the transposase, 4) whether transposon mutagenesis occurs alone or in combination with a sensitized genetic background, 5) the scale of the screen (depending on the number of mice and tumors that are desired for analysis), 6) the duration of the screen (depending on what types of CIS genes are desired at early or late stages of tumorigenesis), 7) the specific methods used for the identification and analysis of transposon insertions, and 8) whether in vivo or ex vivo mutagenesis is desired.