Table 1. Detailed staining protocol for eight plant species.

	Staining solutions
Plant species	KOH	H2O2+NH31	1% HCl	Trypan Blue2
C. rotundifolia	60 min in 2.5% KOH at 80°C	40 min	30 min	60 min at 80°C
L. corniculatus	60 min in 2.5% KOH at 80°C	None	30 min	45 min at 95°C
T. repens	60 min in 2.5% KOH at 80°C	None	30 min	90 min at 90°C
G. sanguineum	30 min in 2.5% KOH at 121°C	None	30 min	75 min at 90°C
F. vesca	48 h in 1.25% KOH at RT3	None	60 min	60 min at 80°C
V. tricolor	60 min in 2.5% KOH at 80°C	None	30 min	75 min at 95°C
T. serpyllum	20 min in 2.5% KOH at 90°C	None	60 min	90 min at 80°C
A. dioica	48 h in 2.5% KOH at RT3	30 min	90 min	90 min at 90°C

¹ 1.5% hydrogen peroxide containing 5 ml/l ammonia.

² Lactic acid containing 63 ml/l glycerol, 63 ml/l water, and 0.02% Trypan Blue.

³ Room temperature.