Skip to main content
. 2018 Dec 31;13(12):e0209862. doi: 10.1371/journal.pone.0209862

Fig 5. zCFTR shows different responses to ATP and phosphate analogues.

Fig 5

(A) Normalized current decay upon washing out 1 mM ATP and 4 mM MgPPi shows almost unaltered relaxation time constant (0.52 ± 0.06 s, N = 3) as compared with washing out 2 mM ATP (0.68 s in Fig 4A) for WT-zCFTR. Similarly, removal of 1 mM ATP and 4 mM MgPPi on WT-nzCFTR yields a time constant of 0.58 ± 0.02 s (N = 3). In contrast, the same experiments on WT-hCFTR yield a current decay time constant of 31.3 ± 3.2 s (N = 8). (B) A real time recording showing that 2 mM dATP elicits a similar macroscopic current as 2 mM ATP for zCFTR. Of note, a previous report showed an increase of Po from 0.45 (2 mM ATP) to 0.82 (2 mM dATP) for human WT-CFTR [51]. (C) A real time recording of WT-zCFTR shows a slight decrease of the macroscopic current upon switching the ligand from 2 mM ATP to 50 μM P-ATP. In contrast, switching from 2.75 mM ATP to 50 μM P-ATP results in ~ 2-fold current increase in hCFTR [49].