Table 1. Primers for candidate reference genes.
| Symbol | Accession | Name | Primer Sequences (5'-3') | Am (bp) | |
|---|---|---|---|---|---|
| HPRT | NM_000194 | Hypoxanthine phosphoribosyl-transferase | F: CCTGGCGTCGTGATTAGTGAT | R:AGACGTTCAGTCCTGTCCATAA | 131 |
| Rpl13A | NM_012423 | Ribosomal protein L13A | F:GCCCTACGACAAGAAAAAGCG | R:TACTTCCAGCCAACCTCGTGA | 117 |
| 18s rRNA | NR_046235.1 | 18s subunit ribosomal RNA | F:AGAAACGGCTACCACATCCA | R:CACCAGACTTGCCCTCCA | 169 |
| U6 | NR_004394.1 | U6 small nuclear 1 | F:CTCGCTTCGGCAGCACA | R:AACGCTTCACGAATTTGCGT | 94 |
| YWHAZ | NM_001135702 | Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zetapolypeptide | F:CCTGCATGAAGTCTGTAACTGAG | R:GACCTACGGGCTCCTACAACA | 100 |
| PPIA | NM_021130 | peptidylprolyl isomerase A | F: ATGTGTCAGGGTGGTGACTTC | R:GCCATCCAACCACTCAGTCTT | 118 |
| GAPDH | NM_001256799 | Glyceraldehyde-3-phosphate dehydrogenase | F:ACAACTTTGGTATCGTGGAAGG | R:GCCATCACGCCACAGTTTC | 101 |
| ACTB | NM_001101.3 | Beta-actin | F:CGACAGGATGCAGAAGGAG | R:ACATCTGCTGGAAGGTGGA | 137 |
| B2M | NM_004048 | Beta-2 microglobulin | F:TCCAGAAACTAATGGCAGATCCC | R:AATTCCCTACGCTTTGGGTTTT | 163 |
| TBP | NM_003194 | TATA box binding protein | F:TGCACAGGAGCCAAGAGTGAA | R:CACATCACAGCTCCCCACCA | 132 |
F: Forward primer; R: Reverse primer; Am: amplicon size; bp: numer of base pairs.