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. 2018 Dec 31;13(12):e0210087. doi: 10.1371/journal.pone.0210087

Table 2. Ranking order of the candidate reference genes by four programs: geNorm, NormFinder, BestKeeper and ΔCt.

Rank geNorm NormFinder BestKeeper ΔCt
Gene Stability (M) Gene variability Gene CV (%Ct) Gene Mean SD
1 U6/TPB 0.172 GAPDH 0.066 GAPDH 0.44 GAPDH 0.322
2 U6 0.132 TPB 0.95 B2M 0.352
3 GAPDH 0.249 TPB 0.145 U6 1.08 U6 0.359
4 B2M 0.275 B2M 0.147 B2M 1.3 TPB 0.364
5 Rpl13A 0.291 ppIA 0.199 ppIA 1.59 Rpl13 0.393
6 ppIA 0.312 Rpl13A 0.207 Rpl13A 1.61 YWHAZ 0.408
7 YWHAZ 0.357 YWHAZ 0.294 YWHAZ 1.74 ppIA 0.415
8 18s rRNA 0.409 18s rRNA 0.347 18s rRNA 2.77 18s rRNA 0.595

M: the mean expression stability values; CV: the coefficient of variance; Mean SD: mean standard deviation.