Table 2. Ranking order of the candidate reference genes by four programs: geNorm, NormFinder, BestKeeper and ΔCt.
Rank | geNorm | NormFinder | BestKeeper | ΔCt | ||||
---|---|---|---|---|---|---|---|---|
Gene | Stability (M) | Gene | variability | Gene | CV (%Ct) | Gene | Mean SD | |
1 | U6/TPB | 0.172 | GAPDH | 0.066 | GAPDH | 0.44 | GAPDH | 0.322 |
2 | U6 | 0.132 | TPB | 0.95 | B2M | 0.352 | ||
3 | GAPDH | 0.249 | TPB | 0.145 | U6 | 1.08 | U6 | 0.359 |
4 | B2M | 0.275 | B2M | 0.147 | B2M | 1.3 | TPB | 0.364 |
5 | Rpl13A | 0.291 | ppIA | 0.199 | ppIA | 1.59 | Rpl13 | 0.393 |
6 | ppIA | 0.312 | Rpl13A | 0.207 | Rpl13A | 1.61 | YWHAZ | 0.408 |
7 | YWHAZ | 0.357 | YWHAZ | 0.294 | YWHAZ | 1.74 | ppIA | 0.415 |
8 | 18s rRNA | 0.409 | 18s rRNA | 0.347 | 18s rRNA | 2.77 | 18s rRNA | 0.595 |
M: the mean expression stability values; CV: the coefficient of variance; Mean SD: mean standard deviation.