Table 4. Primers for target miRNAs.
Symbol | Accession | Primer Sequences (5'-3') | Am (bp) |
---|---|---|---|
miR-145-5p | MIMAT0000437 | RT:TGGAGCGACCGTGTCGTGGAGTCGGCTAATGGTCGCTCCAAGGGAT | 77 |
F:GACACTCCAGCAGCGGTCCAGTTTTCCCAGGAA | |||
R:ATAGAGCGGTGTCGTGGAGTCGGCTAATGGTC | |||
miR-1-3p | MIMAT0000416 | RT:TGGAGCGACCGTGTCGTGGAGTCGGCTAATGGTCGCTCCAATACAT | 75 |
F: GACACTCCAGCAGCGTGGAATGTAAAGAAGT | |||
R:ATAGAGCGGTGTCGTGGAGTCGGCTAATGGTC | |||
miR-124-3p | MIMAT0000422 | RT:TGGAGCGACCGTGTCGTGGAGTCGGCTAATGGTCGCTCCAGGCATT | 74 |
F:GACACTCCAGCAGCGTAAGGCACGCGGTGA | |||
R:ATAGAGCGGTGTCGTGGAGTCGGCTAATGGTC | |||
miR-205-5p | MIMAT0000266 | RT:TGGAGCGACCGTGTCGTGGAGTCGGCTAATGGTCGCTCCACAGACT | 76 |
F:GACACTCCAGCAGCGTCCTTCATTCCACCGGA | |||
R:ATAGAGCGGTGTCGTGGAGTCGGCTAATGGTC |
RT: retro-transcription primer; F: Forward primer; R: Reverse primer; Am: amplicon size; bp: numer of base pairs.