Fig 4. DCAC promoted inflammasome activation in KCs.

KCs were pre-treated with DCAC at concentrations of 0, 1, 2.5 or 5 mM for 24 h. qPCR and Western blot analyses were done for the expression of inflammasome (NLRP3 and caspase1). (A, B) mRNA levels of the caspase1 and NLRP3, as measured by RT-PCR, and (C) Relative protein levels of NLRP3 and caspase1, as determined by Western blotting. n = 3 in each group.*p<0.05 vs. the controls.