(A) Sera from 124 SSc patients and 41 healthy blood donors were screened for specificity to CarBSA by indirect ELISA. Binding of anti-CarP Ab is expressed as a percentage of the binding obtained with positive control serum from an RA patient. (B) Anti-CarP Ab levels in SSc patients divided by age at observation into five groups: I, ≤44 years, 32 cases; II, 44–53 years, 28 cases; III, 54–59 years, 24 cases; IV, 60–66 years, 17 cases; and V, ≥67 years, 23 cases. Horizontal bars mark the medians and boxes indicate interquartile ranges; outliers (more than 1.5 times the interquartile range) are marked with circles, while extreme outliers (more than 3 times the interquartile range) are marked with asterisks. Kruskal-Wallis Htest, *p = 0.037, **p = 0.014.