Table 2. Sample diversity.
| Sample Name | Cell # at start culture | Culture time (days) | Cell isolation | Storage compound | Storage time (days) | Storage temp. (°C) | Extraction method | RNA total (μg) | A260/A280 | A260/A230 | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 3D Fib-Alg 1 | 5.00E+05 | 3 | Trizol | Trizol | 0 | N/A | Trizol | 7.51 | 1.477 | 0.389 |
| 2 | 3D Fib-Alg 2 | 5.00E+05 | 3 | Trizol | Trizol | 0 | N/A | Trizol | 9.67 | 1.487 | 0.448 |
| 3 | 3D Fib-Alg 3 | 5.00E+05 | 3 | Trizol | Trizol | 0 | N/A | Trizol | 10.42 | 1.495 | 0.424 |
| 4 | 3D Fib 1 | 5.00E+05 | 3 | Trizol | Trizol | 0 | N/A | Trizol | 12.43 | 1.519 | 0.380 |
| 5 | 3D Fib 2 | 5.00E+05 | 3 | Trizol | Trizol | 0 | N/A | Trizol | 12.42 | 1.524 | 0.406 |
| 6 | 3D Fib 3 | 5.00E+05 | 3 | Trizol | Trizol | 0 | N/A | Trizol | 12.55 | 1.553 | 0.394 |
| 7 | Cells 48w 1 | 5.00E+05 | 3 | Trizol | Trizol | 0 | N/A | Trizol | 12.27 | 1.520 | 0.412 |
| 8 | Cells 48w 2 | 5.00E+05 | 3 | Trizol | Trizol | 0 | N/A | Trizol | 14.92 | 1.536 | 0.432 |
| 9 | Cells 48w 3 | 5.00E+05 | 3 | Trizol | Trizol | 0 | N/A | Trizol | 9.84 | 1.467 | 0.403 |
| 10 | Beads 1 | 5.00E+05 | 3 | Trizol | Trizol | 0 | N/A | Trizol | 12.97 | 1.507 | 0.442 |
| 11 | Beads 2 | 5.00E+05 | 3 | Trizol | Trizol | 0 | N/A | Trizol | 12.07 | 1.513 | 0.418 |
| 12 | Beads 3 | 5.00E+05 | 3 | Trizol | Trizol | 0 | N/A | Trizol | 10.39 | 1.484 | 0.411 |
| 13 | Cells 12w 1 | 5.00E+05 | 3 | Trizol | Trizol | 0 | N/A | Trizol | 7.50 | 1.490 | 0.504 |
| 14 | Cells 12w 2 | 5.00E+05 | 3 | Trizol | Trizol | 0 | N/A | Trizol | 8.47 | 1.461 | 0.392 |
| 15 | Cells 12w 3 | 5.00E+05 | 3 | Trizol | Trizol | 0 | N/A | Trizol | 12.52 | 1.541 | 0.433 |
| 16 | I.A3 | 1.00E+05 | 3 | RLT | RLT | 0 | N/A | Rneasy | 2.16 | 2.018 | 1.550 |
| 17 | I.B4 | 1.00E+05 | 3 | RLT | RLT | 7 | -80 | Rneasy | 2.52 | 2.047 | 1.563 |
| 18 | I.B3 | 1.00E+05 | 3 | RLT | RLT | 7 | -20 | Rneasy | 2.46 | 2.053 | 1.237 |
| 19 | I.C4 | 1.00E+05 | 3 | RLT | RLT | 30 | -80 | Rneasy | 2.13 | 2.272 | 0.061 |
| 20 | I.C3 | 1.00E+05 | 3 | RLT | RLT | 30 | -20 | Rneasy | 2.83 | 2.095 | 0.421 |
| 21 | I.A2 | 1.00E+05 | 3 | Trizol | Trizol | 0 | N/A | Trizol | 1.93 | 2.125 | 1.088 |
| 22 | I.B2 | 1.00E+05 | 3 | Trizol | Trizol | 7 | -80 | Trizol | 2.65 | 2.056 | 1.931 |
| 23 | I.B1 | 1.00E+05 | 3 | Trizol | Trizol | 7 | -20 | Trizol | 1.16 | 2.054 | 1.362 |
| 24 | I.C2 | 1.00E+05 | 3 | Trizol | Trizol | 30 | -80 | Trizol | 0.92 | 2.035 | 0.669 |
| 25 | I.C1 | 1.00E+05 | 3 | Trizol | Trizol | 30 | -20 | Trizol | 3.28 | 2.046 | 1.564 |
| 26 | II.A3 | 1.00E+05 | 3 | Trypsin | RLT | 0 | N/A | Rneasy | 2.39 | 2.046 | 1.730 |
| 27 | II.B4 | 1.00E+05 | 3 | Trypsin | RLT | 7 | -80 | Rneasy | 2.73 | 2.105 | 1.345 |
| 28 | II.B3 | 1.00E+05 | 3 | Trypsin | RLT | 7 | -20 | Rneasy | 3.51 | 2.081 | 1.804 |
| 29 | II.C4 | 1.00E+05 | 3 | Trypsin | RLT | 30 | -80 | Rneasy | 1.69 | 2.156 | 0.679 |
| 30 | II.C3 | 1.00E+05 | 3 | Trypsin | RLT | 30 | -20 | Rneasy | 2.59 | 2.092 | 1.031 |
| 31 | III.B4 | 1.00E+05 | 3 | Trypsin | RNAlater | 7 | -80 | Rneasy | 1.47 | 2.160 | 0.991 |
| 32 | III.B3 | 1.00E+05 | 3 | Trypsin | RNAlater | 7 | -20 | Rneasy | 1.99 | 2.059 | 1.261 |
| 33 | III.C4 | 1.00E+05 | 3 | Trypsin | RNAlater | 30 | -80 | Rneasy | 1.24 | 2.134 | 0.597 |
| 34 | III.C3 | 1.00E+05 | 3 | Trypsin | RNAlater | 30 | -20 | Rneasy | 1.42 | 2.126 | 0.587 |
| 35 | III.B2 | 1.00E+05 | 3 | Trypsin | RNAlater | 7 | -80 | Trizol | 0.02 | 27.025 | 0.034 |
| 36 | III.B1 | 1.00E+05 | 3 | Trypsin | RNAlater | 7 | -20 | Trizol | 1.80 | 2.071 | 1.517 |
| 37 | III.C2 | 1.00E+05 | 3 | Trypsin | RNAlater | 30 | -80 | Trizol | 1.21 | 1.969 | 0.990 |
| 38 | III.C1 | 1.00E+05 | 3 | Trypsin | RNAlater | 30 | -20 | Trizol | 2.54 | 2.041 | 1.624 |
| 39 | II.A2 | 1.00E+05 | 3 | Trypsin | Trizol | 0 | N/A | Trizol | 2.88 | 2.021 | 1.540 |
| 40 | II.B2 | 1.00E+05 | 3 | Trypsin | Trizol | 7 | -80 | Trizol | 2.93 | 2.069 | 1.861 |
| 41 | II.B1 | 1.00E+05 | 3 | Trypsin | Trizol | 7 | -20 | Trizol | 1.68 | 2.026 | 1.449 |
| 42 | II.C2 | 1.00E+05 | 3 | Trypsin | Trizol | 30 | -80 | Trizol | 1.42 | 2.045 | 1.706 |
| 43 | II.C1 | 1.00E+05 | 3 | Trypsin | Trizol | 30 | -20 | Trizol | 3.10 | 2.044 | 2.017 |
Samples were obtained from MSC-hTERT (1–15) and UC-MSC (16–43) cultured at different cell densities, stored at different temperatures and time, and using different extraction methods. Yield, A260/280, and A260/A230 ratios are given. N/A; Not Applicable.