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. Author manuscript; available in PMC: 2018 Dec 31.
Published in final edited form as: J Am Chem Soc. 2008 Apr 22;130(20):6616–6623. doi: 10.1021/ja710601d

Figure 3.

Figure 3.

In vitro characterization of SgcC as a two-component, FAD- dependent monooxygenase. (A) HPLC profiles of 3-chloro-β-tyrosine (9, ◊) standard (I), hydrolyzed compounds following incubations of (S)-3- chloro-β-tyrosinyl-S-SgcC2 (7) with SgcC for 10 min (II), 20 min (III), and 60 min (IV), and synthetic 3-chloro-5-hydroxyl-β-tyrosine (12, ♦) standard (V). The other peak in the chromatograms is 4,5-dihydroxy-1,2- dithiane (●) presented in the assay. (B) Time course of SgcC-catalyzed hydroxylation of (S)-3-chloro-β-tyrosinyl-S-SgcC2 (7) as followed by HPLC analysis for the first 20 min. (C) Single-substrate kinetic analysis for SgcC with varying concentration of (S)-3-chloro-β-tyrosinyl-S-SgcC2 (7).