. Author manuscript; available in PMC: 2018 Dec 31.

Published in final edited form as: J Antibiot (Tokyo). 2010 Dec 8;64(1):163–166. doi: 10.1038/ja.2010.150

Table 2.

Summary of biological activities for 1-10: (a) inhibition of Cap-dependent translation by 1, 2, 6 and 9 (μM) and cytotoxicity against MDA-MB-231, Du145, HCT-15 and MCF7 human cancer cell lines by 1-3 (μM)

	IC₅₀
A Translation inhibition^a	1	2	6	9

FF luciferase	0.95 ±0.05	0.33±0.10	30.40 ±2.56	1.31±0.03
Ren luciferase	3.54±0.62	0.39±0.30	>50	7.05±0.80
	IC₅₀
B Cytotoxicity^b	1	2	3

MDA-MB-231	0.3 ±0.02	0.33 ±0.01	2.28±0.07
Du145	1.86 ± 0.06	2.21 ±0.05	>10
HCT-15	0.82 ±0.04	0.86 ±0.03	>10
MCF7	0.28±0.01	0.31 ±0.01	2.47 ±0.07

Abbreviations: FF, firefly; Ren, renilla.

Substrate for the translation inhibition assay was capped-(CAG)₃₃FF/HCV/RenpA51 mRNA.

All other compounds showed no detectable inhibition at 20μm.

All other compounds showed no detectable toxicity at 10 μm.