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. Author manuscript; available in PMC: 2018 Dec 31.
Published in final edited form as: Methods Enzymol. 2009;459:97–112. doi: 10.1016/S0076-6879(09)04605-9

Figure 5.3.

Figure 5.3

Degenerate primer design for PCR-based amplification of PKSE fragments. (A) Location of primers in relation to the pksE and associated genes that together constitute a minimal pksE and cassette from the neocarzinostatin (NCS), C-1027, and calicheamicin (CAL) biosynthetic clusters. (B) The conserved amino acid motifs used to design degenerate primers A to H. Numbers between motifs represent amino acid distance, and the noncoding regions between open reading frames (<10 nt) are denoted by a slash.