Figure 1. Experimental study design.

Subjects underwent two experimental days separated by 12 weeks of endurance cycling training (PRE and POST training experimental day). On each experimental day, subjects performed an acute bout of one‐legged exercise (80% peak workload (PWL) interspersed with 3 × 5 min intervals at 100% PWL). After 4 h of rest, a 2 h euglycemic hyperinsulinaemic clamp (EHC) was initiated. Biopsies (B) in the prior exercised and rested legs were taken immediately before and after EHC. Prior to the PRE experimental day and in the 12th training week, body composition, ${\dot{V}}_{{\mathrm{O}}_2\mathrm{peak}}$ and PWL were determined and fasting plasma glucose and plasma insulin levels were measured.