Figure 4. Phosphorylation and expression of Akt2.

Site‐specific phosphorylation of Akt^Ser473 (A) and Akt^Thr308 (B) and Akt2 protein expression (C) measured by Western blotting in the rested (white bars) and prior exercised leg (black bars) 4 h after acute one‐legged exercise (basal) and after a 2 h EHC (insulin) PRE and POST training. Representative blots (D). Data are expressed as the mean ± SEM. AU, arbitrary units; ME, main effect. ^** P < 0.01 PRE vs. POST training; ^† P < 0.05, rested leg vs. acutely exercised leg.